Device

Part:BBa_K174008:Design

Designed by: The Newcastle 2009 iGEM team   Group: iGEM09_Newcastle   (2009-10-10)

SmtA metallothionein protein with CotC and Gfp fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1234
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1046


Design Notes

This brick can be conveniently integrated to B. subtilis using the homology between cotC fusion and the native copy of cotC on the chromosome. This integration leaves the native cotC intact, resulting in a mixture of native and SmtA fused CotC protein.

Source

The smtA sequence was from E. coli and is at Genbank [http://www.ncbi.nlm.nih.gov/gene/6143825?ordinalpos=3&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum here]

References

  1. Cretì, P., F. Trinchella, et al. "Heavy metal bioaccumulation and metallothionein content in tissues of the sea bream Sparus aurata from three different fish farming systems." Environmental Monitoring and Assessment.
  2. Morby, A. P., J. S. Turner, et al. (1993). SmtB is a metal-dependent repressor of the cyanobacterial metallothionein gene smtA: identification of a Zn inhibited DNA-protein complex. 21: 921-925.
  3. Waldron, K. J. and N. J. Robinson (2009). "How do bacterial cells ensure that metalloproteins get the correct metal?" Nat Rev Micro 7(1): 25-35.